Transcription profiling of irradiated non-adherent anoikis-resistant DU145 and MCF-7 cells and 5-azacytidine-treated non-adherent anoikis-resistant HeLa cells in contrast to control (non-irradiated, non-treated) cells.

ABSTRACT: Radiation and chemotherapy represent standard-of-care cancer treatments, however most treated patients eventually experience tumor recurrence, treatment failure and metastatic dissemination with fatal consequences. To elucidate molecular mechanisms of resistance to radio- and chemo-therapy, we exposed human cancer cell lines (HeLa, MCF-7, and DU145) to clinically relevant doses of 5-azacytidine or ionizing radiation and compared the transcript profiles of all surviving cell subpopulations including non-adherent fraction. Stress-mobilized non-adherent cell fractions differed from other survivors in deregulation of hundreds of genes including those involved in interferon response. Exposure of cancer cells to interferon-gamma but not interferon-beta resulted in development of a heterogeneous

ORGANISM(S): Homo sapiens

SUBMITTER: Lenka Kyjacova 

PROVIDER: E-MTAB-6062 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress