Small RNA-seq analysis of single porcine blastocysts revealed that maternal estradiol-17beta exposure does not affect miRNA isoform (isomiR) expression

ABSTRACT: Small RNA sequencing of 36 porcine embryo samples collected on Day 10 of pregnancy detected miRNA sequences mapping to known and predicted porcine miRNAs, and novel miRNAs highly conserved in human and cattle. A set of highly abundant miRNAs was identified as well as a large number of rarely expressed miRNAs. Due to the high number of generated sequence reads, it was possible to detect a large number of different miRNA isoforms (isomiRs). Potentially embryo-specific miRNAs were identified by comparing the data with small RNA-Seq data sets from porcine endometrium and a search in databases for miRNA tissue expression. The identification of 140 novel miRNAs in addition to 173 known porcine miRNAs (miRBase21) showed the benefit of our small RNA analysis pipeline. This benefit has a big impact

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Sus scrofa

SUBMITTER: Jochen Bick 

PROVIDER: E-MTAB-6201 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress