Transcriptome of murine DC-like XS52 cells stably overexpressing miRNA 223 compared to control XS52 cells

ABSTRACT: Cells of the DC-like cell line XS52 (Xu et al., 1995, J Invest Dermatol. 1995 Dec;105(6):831-6) were lentivirally transduced with a copGFP reporter (pGreenFire) or the mmu-miR-223 gene locus (both from SBI Biosciences, Palo Alto, CA) and cultured in the presence of puromycin to select for transductants as described (Bollmann et al., 2014, Nucleic Acids Res. 42, 12555-12569). Each 20 µg of total RNA derived from the two transduced XS52 sublines was reverse-transcribed using the SuperScript Plus Direct cDNA Labeling System (Invitrogen, Carlbad, CA), with Alexa Fluor 555-aha-dUTP or Alexa Fluor 647-aha-dUTP to differentially label cDNA of either group. Derived fluorescence-labeled cDNA was purified and analysed for quality and yield (Nanodrop ND-100; Peqlab, Erlangen, Germany). Prehybridized

ORGANISM(S): Mus musculus

SUBMITTER: Hartmut Kleinert 

PROVIDER: E-MTAB-6369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress