Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of miRNA in mouse models of melanoma comparing niches of tumour tissue to adjacent normal tissue

ABSTRACT: This experiment focused on the miRNA expressions within the tissues specific niches of mice afflicted with melanoma, compared to littermates without malignancy. BRafCA/ PtenloxP/ Tyr::CreERT2 or genetically matched littermates without Cre recombinase were treated with 2µl 4-hydroxytamoxifen (5mM), every other day, for on days 0, 2, and 4, on the shaved right flank of the abdomen of the animal. Mice slowly grow melanoma at the treated site, and some metastases distal to treatment location (eg. Ear or opposite side of abdomen). Endpoint criteria was designated as a Samples were taken when tumors reached 1.5mm diameter. Total RNA was isolated from tumors or tamoxifen treated healthy skin, skin adjacent to the treatment site, or the ear ( representing a metastatic site). Total RNA was prepared with FLAG-TAG kit and miRNA microarray 4.0 was performed.

ORGANISM(S): Mus musculus

SUBMITTER: Robert Zeiser

PROVIDER: E-MTAB-6373 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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