Gene expression time series of Arabidopsis thaliana Col-0 and rcd1 genotypes in ozone and control

ABSTRACT: Wild-type Columbia (Col-0) and rcd1-1 seeds were sown on 1:1 peat:vermiculite mixture, stratified for 2 days and grown in controlled environment chambers (Weiss Bio1300; Weiss Gallenkamp, (http://www.weiss-gallenkamp.com/) with 12-h/12-h day/night cycle, temperature 22 C/19 C, relative humidity 70%/90%. 1-week old plants were transplanted into individual pots (5x5cm) and subirrigated twice a week. O3 experiments (6 hours of 350 nL L-1) were performed with 3-week old plants. Control and O3-treated plants (x-10 individuals) were collected at 0, 1, 2, 4, 8 and 24h after the start of the O3 treatment. The experiment was repeated 3 times, in addition to which a fourth identical repeat was used as the common reference RNA. cDNA synthesis, sample labeling, microarray hybridization, washing of the

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jarkko Salojarvi 

PROVIDER: E-MTAB-662 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress