Characterization of a tetra-culture system combining lung alveolar epithelial cells, endothelial cells, macrophages, and mast cells in a three-dimensional orientation

ABSTRACT: To more accurately model inhalation toxicity in vitro, we developed a tetra-culture system that combines lung alveolar epithelial cells, endothelial cells, macrophages, and mast cells in a three-dimensional orientation. We characterized the influence of the added complexity, using network perturbation analysis and gene expression data, to gain an insight into the steady-state profile of the assembled complete three-dimensional model using all four cell types and of simpler models of one, two, or three of the cell types. Gene expression data were analyzed using cause-and-effect biological network models, together with a quantitative network-scoring algorithm to determine the biological impact of co-culturing the various cell types. In the assembled tetra-culture, macrophages appeared to be

ORGANISM(S): Homo sapiens

SUBMITTER: Alain Sewer 

PROVIDER: E-MTAB-7072 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress