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Custom design microarray for molecular drug susceptibility testing and strain typing of tuberculosis

ABSTRACT: After performing multiplex PCR, we analysed extracted DNA (500 ng ssDNA) from 9 Mycobacterium tuberculosis clinical isolates to detect multidrug resistance. In addition, a mixed strain situation was simulated by mixing wild type Mtb CDC1551 (20 ng) with 4 concentrations of Mtb mutant DNA (1 ng, 250 pg, 62.5 pg, and 15.6 pg), which is equivalent to relative concentrations of 5%, 1.25%, 0.31% and 0.08% Mtb mutant DNA.

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Tom Venken

PROVIDER: E-MTAB-7610 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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