RNA-seq of isolated tumor cells from high grade serous ovarian cancer (HGSOC) patients

ABSTRACT: Tumor cells were isolated from solid tumors or ascites from HGSOC patients and enriched for EpCAM expression by antibody magnetic beads (affinity)-based methods and large RNAs (> 200 nt) sequenced (50 bp paired ends) after rRNA depletion (RiboZero). Expression differences between patients with miliary and non-miliary peritoneal tumor spreading were used for a search for new targeted therapies and for biological annotion.

INSTRUMENT(S): QIAcube (Qiagen), epMotion 5075 automated pipetting system (Eppendorf, Hamburg, Germany), Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Dietmar Pils 

PROVIDER: E-MTAB-7674 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress