ScRNAseq of Cyp11a1-expressing cells from mouse melanoma model derived tumors

ABSTRACT: Tumours induce de novo steroidogenesis in immune cells. We wanted to define the gene expression identity of intratumoural steroidogenic immune cells and compare them with the transcriptome with non-steroidogenic cells. Cyp11a1 expression is a faithful biomarker of de novo steroidogenesis (i.e. Cyp11a1+ cells are steroidogenic and Cyp11a1- cells are non-steroidogenic). We inoculated B16-F10 tumor in Cyp11a1-mCherry reporter mice, enriched and purified intratumoral Cyp11a1-mCherry+ and Cyp11a1-mCherry- cells by cell sorting into 96-well plates [with a ratio of 79:15 (mCherry+ : mCherry-) cells per plate] and performed scRNA-seq using SMART-Seq2 platform.

INSTRUMENT(S): BD Influx, Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Krzysztof Polanski 

PROVIDER: E-MTAB-8509 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress