Genome-wide identification of transcriptional start sites in the phytopathogen Dickeya dadantii 3937 based on differential RNA-Seq (dRNA-Seq)

ABSTRACT: In this study transcriptional start sites (TSS) for the phytopathogen Dickeya dadantii 3937 were determined based on differential RNA-seq (dRNA-seq) as previously described (Sharma and Vogel, 2014). RNAs from E-MTAB-541 (Jiang et al. 2015) were pooled into four samples S1 to S4 reflecting a variety of environmental conditions. Each of them resulted in a combination of stress conditions (pH, NaCl, H2O2) and growth conditions: exponential phase with stress (S1), exponential phase without stress (S2), stationary phase with stress (S3), stationary phase without stress (S4). Those samples were then supplied to Vertis Biotechnologie AG for TEX treatment and Illumina sequencing. Briefly, ribosomal RNA molecules were depleted from the total RNA samples using the Ribo-Zero rRNA Removal Kit for bac

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Dickeya dadantii

SUBMITTER: Raphaël Forquet 

PROVIDER: E-MTAB-9075 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress