Transcriptional profiling of fruit flies overexpressing the Atg1 gene

ABSTRACT: Overexpression of Atg1 using either weaker CGGAL4 or stronger HRGAL4 driver. Both drivers have same expression pattern and are specific for intestine, Malpighian tubules and fat body of the fruit fly Drosophila melanogaster. Weaker Atg1 overexpression results in longer lifespan whereas stronger Atg1 overexpression shortens lifespan compared to controls. Overexpressor lines were combined with tubGAL80ts, which is a temperature sensitive GAL4 repressor. This enabled induction of Atg1 overexpression in day-2 adult fly, thereby bypassing development and any potential developmental effects that Atg1 might have. Fly eggs of appropriate genotype were raised under standard density at 18C and then emerged flies were switched to 27C at day 2 of adulthood for Atg1 overexpression, and were kept at 27C

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Daniela Wieser 

PROVIDER: E-MTAB-9391 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress