Aging of myelinating glial cells predominantly affects lipid metabolism and immune response pathways

ABSTRACT: In order to uncover the biological processes affected in glial cells by aging, we analyzed microarray gene expression of the Schwann cell-rich mouse sciatic nerve at 17 time-points throughout life, from day of birth until senescence. In addition, we combined these data with the microarray gene expression data of myelin 56 day-old mouse mutants carrying deletions of either Pmp22, SCAP or Lpin1. Seven mice were dissected per developmental or aging time-point. Both sciatic nerves were isolated from each mouse and tissues were pooled per time-point to extract total RNA. For the mutants and their wild-type littermates, sciatic nerves were dissected from three mice per genotype. Tissues were not pooled, which generated triplicates per genotype. Total RNA was extracted, purified and quality-cont

ORGANISM(S): Mus musculus

DISEASE(S): normal

SUBMITTER: Roman Chrast 

PROVIDER: E-MTAB-944 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress