Transcription profiling of mouseWT vs CD137L-/- GC B cells

ABSTRACT: WT and CD137L-/- mice of 8-12 weeks old were immunized intra-peritoneally with 50 µg chicken gamma- globulin conjugated to 4-hydroxy-3-nitrophenylacetyl (NP-CG) in alum or infected intranasally with 25 hemagglutinin units of influenza virus strain A/NT/60/68 in 50 µl HBSS. Nine days after immunization or infection, B cells were enriched from pooled spleens and lymph nodes of 4 mice per test group by means of magnetic labelled bead cell separation (MACS) using anti-mouse BD ImagTM CD45R/B220-particles DM. The B220+ MACS-sorted populations were stained with anti-GL7-FITC and anti-CD19-PE to sort CD19+GL7+ GC and CD19+GL7- non-GC B lymphocytes by flow cytometry on a FACSAria (BD). Around 1 million cells of each sample were obtained. These cells were spun down and the pellet was frozen in li

ORGANISM(S): Mus musculus

SUBMITTER: Victor Peperzak 

PROVIDER: E-NCMF-29 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress