Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Streptococcus pneumoniae after incubation with host respiratory tract epithelial cells

ABSTRACT: In order to study pneumococcal gene expression changes after incubation with host epithelial cells, logarithmic-phase grown S. pneumoniae strains (82 and R6) were incubated with host epithelial cells (A549 and ETEC) in T75 cell culture flasks at an MOI range of 10:1 ~ 50:1. After incubation for 1 h or 3 h, bacterial solution was removed and 7 ml of host cell lysis buffer which can remove host cells and stablize bacterial RNA was added immediately. Bacteria incubated with antibiotic-free media (MEM or DMEM) with 1% (v/v) FBS for the same time periods were used as medium controls. The RNA samples were isolated with RiboPure Bacteria kit (Ambion) or RNeasy Minikit (Qiagen), and purified with DNase I treatment. cDNA samples were synthesized and labeled with biotin-dUTP (samples) and fluorescein-dUTP (Enzo) (medium controls), respectively, by using a LabelStar Array kit (Qiagen). Divergent labeled samples were hybridized to the same microarray slide by using a Two-Color Nucleic Acid Microarray Toolkit (Invitrogen). The scattered light signals of Resonance Light Scattering (RLS) particles Ag and Au were captured with a GSD-501 RLS imager using an ICS-501 (version 2.3) Image Capture System (Invitrogen). The gridding of spots was performed with ArrayVision 8.3 (Imaging Research).

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Xin-Ming Song

PROVIDER: E-TABM-187 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Microarray analysis of Streptococcus pneumoniae gene expression changes to human lung epithelial cells.

Song Xin-Ming XM Connor Wayne W Jalal Shakiba S Hokamp Karsten K Potter Andrew A AA

Canadian journal of microbiology 20080301 3

Streptococcus pneumoniae infection starts from the respiratory tract where interaction with host epithelial cells occurs. To gain more insights on pneumococcal pathogenesis, an oligonucleotide (oligo)-based microarray was used to investigate gene expression changes of one serotype 3 encapsulated pathogenic S. pneumoniae strain 82 and one unencapsulated avirulent S. pneumoniae strain R6 upon exposure to human lung epithelial cells (A549) for 1 and 3 h, respectively. We observed that genes associa ...[more]

PMID: 18388990

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Dataset Information

Transcription profiling of Streptococcus pneumoniae after incubation with host respiratory tract epithelial cells

Publications

Microarray analysis of Streptococcus pneumoniae gene expression changes to human lung epithelial cells.

Similar Datasets

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