Proteomics

Dataset Information

0

The E. coli type III secreted effectors NleB and EspL interact with the microtubule associated protein ensconsin to disrupt vesicle trafficking, part 1


ABSTRACT: Enteropathogenic and enterohaemorrhagic E. coli (EPEC and EHEC) translocate a set of type III effector proteins into host cells that are critical for bacterial virulence. These effectors subvert normal host pathways by interacting with a variety of targets within the cell, but the binding partners and mechanism of action of the majority of effectors are not understood. We identified the microtubule associated protein, ensconsin, as a novel target for two EPEC/EHEC effectors. We found that the secreted effectors NleB and EspL bind host ensconsin and work synergistically to paralyze kinesin-based intracellular vesicular transport. Our findings demonstrate that EPEC/EHEC encode multiple effectors that control intracellular vesicle movement and suggest a simple strategy for broad-based immobilization of host cells by pathogens.

INSTRUMENT(S): LTQ FT, LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

SUBMITTER: Leonard Foster  

LAB HEAD: Leonard James Foster

PROVIDER: PXD002933 | Pride | 2016-12-22

REPOSITORIES: Pride

altmetric image

Publications

Quantitative Mass Spectrometry Identifies Novel Host Binding Partners for Pathogenic Escherichia coli Type III Secretion System Effectors.

Law Robyn J RJ   Law Hong T HT   Scurll Joshua M JM   Scholz Roland R   Santos Andrew S AS   Shames Stephanie R SR   Deng Wanyin W   Croxen Matthew A MA   Li Yuling Y   de Hoog Carmen L CL   van der Heijden Joris J   Foster Leonard J LJ   Guttman Julian A JA   Finlay B Brett BB  

Journal of proteome research 20160328 5


Enteropathogenic and enterohemorrhagic Escherichia coli cause enteric diseases resulting in significant morbidity and mortality worldwide. These pathogens remain extracellular and translocate a set of type III secreted effector proteins into host cells to promote bacterial virulence. Effectors manipulate host cell pathways to facilitate infection by interacting with a variety of host targets, yet the binding partners and mechanism of action of many effectors remain elusive. We performed a mass s  ...[more]

Similar Datasets

2016-09-01 | GSE13403 | GEO
| E-GEOD-13403 | biostudies-arrayexpress
2017-09-11 | PXD006810 | Pride
| E-GEOD-73986 | biostudies-arrayexpress
| E-GEOD-67947 | biostudies-arrayexpress
2009-06-26 | GSE14069 | GEO
| 2184564 | ecrin-mdr-crc
| E-GEOD-23032 | biostudies-arrayexpress
| E-GEOD-67949 | biostudies-arrayexpress
| E-GEOD-8889 | biostudies-arrayexpress