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Serine ubiquitination of SNAREs generates bacterial vacuoles and prevents their lysosomal fusion

ABSTRACT: Here we show that Legionella SidE effectors mediate phosphoribosyl-linked serine ubiquitination (PR-Ub) of the autophagic SNARE proteins STX17 and SNAP29. On the one hand, PR-Ub modification of STX17 enhances its interaction with ATG14L, which drives the recruitment of ER membranes to the bacterial vacuole in a PI3K-dependent manner and promotes the formation of bacterial replicative vacuoles. On the other hand, PR-Ub of SNAP29 inhibits the formation of the autophagosomal SNARE complex (STX17-SNAP29-VAMP8) by steric hindrance and thus prevents the fusion of STX17 + bacterial vacuoles with lysosomes.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Anshu Bhattacharya

LAB HEAD: Ivan Dikic

PROVIDER: PXD058184 | Pride | 2025-05-14

REPOSITORIES: Pride

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			Action	DRS
	20220216_RR_AB_STX17_TMT_1.pdResult	Other
	20220216_RR_AB_STX17_TMT_1.pdResultView	Other
	20220217_RR_AB_STX17_TMT_4.pdResult	Other
	20220217_RR_AB_STX17_TMT_4.pdResultView	Other
	20220217_RR_AB_STX17_TMT_5.pdResult	Other

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