Transcription profiling of brain and spleen from rhesus macaque to validate two oligonucleotide array

ABSTRACT: We constructed a database containing 248,000 exon sequences from 23,000 human RefSeq genes and compared each human exon with its best matching sequence in the January 2005 version of the rhesus genome project list of 486,000 DNA contigs. Best matching rhesus exon sequences for each of the 23,000 human genes were then concatenated in the proper order and orientation to produce a rhesus “virtual transcriptome.” Microarray probes were designed, one per gene, to the region closest to the 3? untranslated region (UTR) of each rhesus virtual transcript. Each probe was compared to a composite rhesus/human transcript database to test for cross-hybridization potential yielding a final probe set representing 18,296 rhesus/human gene orthologs, including transcript variants, and over 17,000 distinct g

ORGANISM(S): Macaca mulatta

SUBMITTER: Sean Proll 

PROVIDER: E-TABM-189 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress