Transcription profiling of Dictyostelium discoideum treated with mercury - a systems biology assessment of the model eukaryote Dictyostelium discoideum unveils compensatory responses and a critical role of the amino acid metabolic balance in the early phase of mercury induced cyto-toxicity

ABSTRACT: Gene expression alone is not able to characterize the physiological status of pollutant-challenged organisms. However, when transcriptomics is integrated with functional information, it becomes an extremely powerful tool, able to distinguish between compensatory and non-protective cytotoxic responses. Here we present the implementation of a systems biology approach based on transcriptomics, metabolomics, targeted proteomics, and functional cellular tests to assess the global effects of mercury - a diffuse environmental contaminant and a cell poison - on the model eukaryote Dictyostelium discoideum. Two different exposure levels were compared, a sub-lethal condition and the lowest observable lethal dose. DNA microarrays and gene ontology analysis indicated quantitative and qualitative diffe

ORGANISM(S): Dictyostelium discoideum

SUBMITTER: Gareth Bloomfield 

PROVIDER: E-TABM-710 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress