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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of E. coli wild type and fis or hns transcription factor mutants in CSH50, LZ41 and LZ54 strains during different growth phases, with relaxation and hypernegative supercoiling and after norflaxacin treatment

ABSTRACT: Comparison of transcript profiles of E. coli CSH50 wild-type and fis or hns mutant cells to distinguish between the genes expressed either in the presence or absence of transcriptional regulators FIS and H-NS during different growth phases. Samples were taken in Mid-exponential phase (ME), Transition to Stationary (TS) and Late Stationary phase (LS).

Effect of FIS and H-NS on gene expression at relaxed and hypernegative supercoiling level was also studied using LZ41 and LZ54 strains. LZ41 and LZ54 strains contain drug-resistant alleles of different topoisomerase genes. In the LZ41 strain norfloxacin treatment strongly relaxes DNA, whereas in the LZ54 strain the same treatment generates high negative supercoiling (Khodursky et al., 1995, PNAS 92:11801-5; Ziechedrich et al, 1997, Genes Dev. 11:2580-92).

ORGANISM(S): Escherichia coli

SUBMITTER: Georgi Muskhelishvili

PROVIDER: E-TABM-86 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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