Chromatin immunoprecipitation of Lmd protein in Drosophila melanogaster during embryonic development
Ontology highlight
ABSTRACT: Genomewide mapping of Drosophila Lmd protein binding during embryonic development. Two consecutive timepoints (6-8 and 8-10 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Lmd protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.
Project description:Genomewide mapping of D. melanogaster Biniou protein binding during embryonic development. Four consecutive timepoints (6-8, 8-10, 10-12, 12-14 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Biniou protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.
Project description:Expression profiling of Drosophila Lmd (lame-duck) homozygous mutant embryos in a timecourse of embryogenesis. Five one-hour timepoints were assayed (5-11 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together on cDNA arrays.
Project description:Genomewide mapping of D. melanogaster Bagpipe protein binding during embryonic development. One time point (6-8 hrs after egg-laying) were assayed in four independent repeats. Two different antibodies were used to precipitate the Bagpipe protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.
Project description:Genomewide mapping of Drosophila Tinman protein binding during embryonic development. Three consecutive timepoints (2-4, 4-6, 6-8 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Tinman protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.
Project description:Genomewide mapping of D. melanogaster Biniou protein binding during embryonic development. Three consecutive timepoints (6-8, 8-10, 10-12 hrs after egg-laying) were assayed in two independent repeats each. Two different antibodies were used to precipitate the Biniou protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to high density Affymetrix GeneChip Drosophila Tiling 1.0R array.
Project description:Genomewide mapping of D. melanogaster Tinman protein binding during embryonic development. Three consecutive timepoints (2-4, 4-6, 6-8 hrs after egg-laying) were assayed in two independent repeats each. Two different antibodies were used to precipitate the Tinman protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to high density Affymetrix GeneChip Drosophila Tiling 1.0R array.
Project description:Genomewide mapping of Drosophila Twist protein binding during embryonic development. Two consecutive timepoints (2-4 and 4-6 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Twist protein. Additionally, preimmune-serum was used as a control. The enriched DNA was hybridized to custom designed tiling arrays optimized for assaying all E-box motifs outside repetitive or coding regions of the Drosophila melanogaster genome (average gap size = 290 bps).
Project description:Genomewide mapping of Drosophila Mef2 protein binding during embryonic development. Five consecutive timepoints (2-4, 4-6, 6-8, 8-10, 10-12 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Mef2 protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.
Project description:Genomewide mapping of D. melanogaster Lame duck protein binding at 6-8hrs after egg laying. Two different rabbit antibodies were used to precipitate the Lmd protein isoform in biological replicates. Additionally a rabbit preimmune-serum was used as a control. The enriched DNA was hybridized to high density Affymetrix GeneChip Drosophila Tiling 1.0R array.
Project description:Expression profiling of Drosophila Mef2 homozygous mutant embryos in a timecourse of embryogenesis. Twelve one-hour timepoints were assayed (5-16 hrs, 18-19 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together on cDNA arrays.