Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals.

ABSTRACT: X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein crystals, the experiment has proven difficult to perform, especially on radiation-sensitive proteins. Here, the development of serial X-ray fluorescence holography to allow the direct recording of hologram patterns before the onset of radiation damage is reported. By combining a 2D hybrid detector and the serial data collection used in serial protein crystallography, the X-ray fluorescence hologram can be directly recorded in a fraction of the measurement time needed for conventional XFH measurem

SUBMITTER: Ang AKR 

PROVIDER: S-EPMC10000799 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature