Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes.

ABSTRACT: Glia, and in particular astrocytes, are one of the major players in neurological and neuroinflammatory disorders. Here, we present a protocol to efficiently generate inflammatory responsive astrocytes from human induced pluripotent stem cells in a monolayer culture. We describe steps for neural differentiation to reach a homogeneous population of neural progenitor cells, followed by their differentiation into neural/glial progenitors. Finally, we detail enrichment to a 90% pure inflammatory responsive astrocyte population. For complete details on the use and execution of this protocol, please refer to Giordano et al.¹.

SUBMITTER: Giordano AMS 

PROVIDER: S-EPMC10009719 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature