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Protocol for isolation of melanopsin and rhodopsin in the human eye using silent substitution.

ABSTRACT: Melanopsin-mediated visual and non-visual functions are difficult to study in vivo. To isolate melanopsin responses, non-standard light stimulation instruments are required, with at least as many primaries as photoreceptor classes in the eye. In this protocol, we describe the physical light calibrations of the display instrumentation, control of stimulus artefacts, and correction of individual between-eye differences in human observers. The protocol achieves complete photoreceptor silent substitution in psychophysical, pupillometry, and electroretinographic experiments for probing melanopsin, rod, and cone function. For complete details on the use and execution of this protocol, please refer to Uprety et al. (2022).¹.

SUBMITTER: Nugent TW

PROVIDER: S-EPMC10011832 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Protocol for isolation of melanopsin and rhodopsin in the human eye using silent substitution.

Nugent Thomas W TW Carter Drew D DD Uprety Samir S Adhikari Prakash P Feigl Beatrix B Zele Andrew J AJ

STAR protocols 20230307 1

Melanopsin-mediated visual and non-visual functions are difficult to study in vivo. To isolate melanopsin responses, non-standard light stimulation instruments are required, with at least as many primaries as photoreceptor classes in the eye. In this protocol, we describe the physical light calibrations of the display instrumentation, control of stimulus artefacts, and correction of individual between-eye differences in human observers. The protocol achieves complete photoreceptor silent substit ...[more]

PMID: 36892996

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