Evaluation of a real-time mobile PCR device (PCR 1100) for the detection of the rabies gene in field samples.

ABSTRACT:

Background

The Philippines is ranked among the top countries with 200-300 annual deaths due to rabies. Most human rabies cases have been reported in remote areas, where dog surveillance is inadequate. Therefore, a strategy to effectively improve surveillance in remote areas will increase the number of detections. Detecting pathogens using portable real-time reverse transcription-polymerase chain reaction (RT-PCR) has the potential to be accepted in these areas. Thus, we aimed to develop an assay to detect the rabies virus (RABV) genome by combining the robust primer system LN34 with the PicoGene PCR1100 portable rapid instrument targeting RABV RNA (PCR1100 assay).

Methods

Procedures were optimised using an LN34 primer/probe set, KAPA3G Plant PCR Kit (KAPA Biosystems), FastGe

SUBMITTER: Demetria C 

PROVIDER: S-EPMC10020757 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature