T lymphocyte characteristics and immune repertoires in the epicardial adipose tissue of heart failure patients.

ABSTRACT:

Background

Epicardial adipose tissue (EAT) acts as an active immune organ and plays a critical role in the pathogenesis of heart failure (HF). However, the characteristics of immune cells in EAT of HF patients have rarely been elucidated.

Methods

To identify key immune cells in EAT, an integrated bioinformatics analysis was performed on public datasets. EAT samples with paired subcutaneous adipose tissue (SAT), heart, and peripheral blood samples from HF patients were collected in validation experiments. T cell receptor (TCR) repertoire was assessed by high-throughput sequencing. The phenotypic characteristics and key effector molecules of T lymphocytes in EAT were assessed by flow cytometry and histological staining.

Results

Compared with SAT, EAT was enriched for im

SUBMITTER: Zhang XZ 

PROVIDER: S-EPMC10027920 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature