CeO₂-Co₃O₄ nanocomposite with oxidase-like activity for colorimetric detection of ascorbic acid.

ABSTRACT: A CeO₂-Co₃O₄ nanocomposite (NC) was prepared and characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy and X-ray diffraction. The obtained CeO₂-Co₃O₄ NC displayed biomimicking oxidase-like activity, which can catalytically oxidize the 3, 3', 5, 5'-tetramethylbenzidine (TMB) substrate from colorless to the blue oxidized TMB (ox-TMB) product with a characteristic absorption peak at 652 nm. When ascorbic acid (AA) was present, ox-TMB would be reduced, resulting in a lighter blue and lower absorbance. On the basis of these facts, a simple colorimetric method for detection of AA was established with a linear relationship ranging from 1.0 to 500 μM and a detection limit o

SUBMITTER: Yang J 

PROVIDER: S-EPMC10043987 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature