NF-κB Activator 1 downregulation in macrophages activates STAT3 to promote adenoma-adenocarcinoma transition and immunosuppression in colorectal cancer.

ABSTRACT:

Background

Adenoma-adenocarcinoma transition is a key feature of colorectal cancer (CRC) occurrence and is closely regulated by tumor-associated macrophages (TAMs) and CD8⁺ T cells. Here, we investigated the effect of the NF-κB activator 1 (Act1) downregulation of macrophages in the adenoma-adenocarcinoma transition.

Methods

This study used spontaneous adenoma-developing Apc^Min/+, macrophage-specific Act1-knockdown (anti-Act1), and Apc^Min/+; anti-Act1 (AA) mice. Histological analysis was performed on CRC tissues of patients and mice. CRC patients' data retrieved from the TCGA dataset were analyzed. Primary cell isolation, co-culture system, RNA-seq, and fluorescence-activated cell sorting (FACS) were used.

Results

By TCGA and TISIDB an

SUBMITTER: Wang S 

PROVIDER: S-EPMC10053426 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature