Dataset Information

High microbiome variability in pediatric tracheostomy cannulas in patients with similar clinical characteristics.

ABSTRACT:

Objectives

To evaluate the bacterial microbiome found in tracheostomy cannulas of a group of children diagnosed with glossoptosis secondary to Robin Sequence (RS), and its clinical implications.

Methods

Pediatric patients were enrolled in the study at the time of the cannula change in the hospital. During this procedure, the removed cannula was collected and stored for amplicon sequencing of 16s rRNA. DNA extraction was performed using DNeasy PowerBiofilm Kit (QIAGEN® ‒ Cat nº 24000-50) while sequencing was performed with the S5 (Ion S5™ System, Thermo Fisher Scientific), following Brazilian Microbiome Project (BMP) protocol.

Results

All 12 patients included in the study were using tracheostomy uncuffed cannulas of the same brand, had tracheostomy performed for over 1-year and had used the removed cannula for approximately 3-months. Most abundant genera found were Aggregatibacter, Pseudomonas, Haemophilus, Neisseria, Staphylococcus, Fusobacterium, Moraxella, Streptococcus, Alloiococcus, and Capnocytophaga. Individual microbiome of each individual was highly variable, not correlating to any particular clinical characteristic.

Conclusion

The microbiome of tracheostomy cannulas is highly variable, even among patients with similar clinical characteristics, making it challenging to determine a standard for normality.

SUBMITTER: Kuhl LP

PROVIDER: S-EPMC10071543 | biostudies-literature | 2023 Mar-Apr

REPOSITORIES: biostudies-literature

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Publications

High microbiome variability in pediatric tracheostomy cannulas in patients with similar clinical characteristics.

Kuhl Leonardo Palma LP Marostica Paulo José Cauduro PJC Macedo Alexandre José AJ Kuhl Gabriel G Siebert Marina M Manica Denise D Sekine Leo L Schweiger Cláudia C

Brazilian journal of otorhinolaryngology 20220520 2

<h4>Objectives</h4>To evaluate the bacterial microbiome found in tracheostomy cannulas of a group of children diagnosed with glossoptosis secondary to Robin Sequence (RS), and its clinical implications.<h4>Methods</h4>Pediatric patients were enrolled in the study at the time of the cannula change in the hospital. During this procedure, the removed cannula was collected and stored for amplicon sequencing of 16s rRNA. DNA extraction was performed using DNeasy PowerBiofilm Kit (QIAGEN® ‒ Cat nº 240 ...[more]

PMID: 35680554

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Dataset Information

High microbiome variability in pediatric tracheostomy cannulas in patients with similar clinical characteristics.

Objectives

Methods

Results

Conclusion

Publications

High microbiome variability in pediatric tracheostomy cannulas in patients with similar clinical characteristics.

Similar Datasets

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