Ab initio reconstruction of small angle scattering data for membrane proteins in copolymer nanodiscs.

ABSTRACT:

Background

Small angle scattering techniques are beginning to be more widely utilised for structural analysis of biological systems. However, applying these techniques to study membrane proteins still remains problematic, due to sample preparation requirements and analysis of the resulting data. The development of styrene-maleic acid co-polymers (SMA) to extract membrane proteins into nanodiscs for further study provides a suitable environment for structural analysis.

Methods

We use small angle neutron scattering (SANS) with three different contrasts to determine structural information for two different polymer nanodisc-incorporated proteins, Outer membrane protein F (OmpF) and gramicidin. Ab initio modelling was applied to generate protein/lipid structures from the S

SUBMITTER: Morrison KA 

PROVIDER: S-EPMC10074903 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature