Evaluation of hetero-multivalent lectin binding using a turbidity-based emulsion agglutination assay.

ABSTRACT: Lectin hetero-multivalency, binding to two or more different types of ligands, has been demonstrated to play a role in case of both LecA (a Pseudomonas aeruginosa adhesin) and Cholera Toxin subunit B (a Vibrio cholerae toxin). In order to screen the ligand candidates that involve in hetero-multivalent binding from large molecular libraries, we present a turbidity-based emulsion agglutination (TEA) assay that can be conducted in a high-throughput format using the standard laboratory instruments and reagents. The benefit of this assay is that it relies on the use of emulsions that can be formed using ultrasonication, minimizing the bottleneck of substrate surface functionalization. By measuring the change in turbidity, we could quantify the lectin-induced aggregation rate of oil droplets to determine the relative binding strength between different ligand combinations. The TEA results are consistent with our prior binding results using a nanocube sensor. The developed TEA assay can serve as a high-throughput and customizable tool to screen the potential ligands involved in hetero-multivalent binding.

SUBMITTER: Worstell NC 

PROVIDER: S-EPMC10079213 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature