Calcium dynamics and modulation in carrot somatic embryogenesis.

ABSTRACT: Free calcium (Ca²⁺) is a pivotal player in different in vivo and in vitro morphogenic processes. In the induction of somatic embryogenesis, its role has been demonstrated in different species. In carrot, however, this role has been more controversial. In this work, we developed carrot lines expressing cameleon Ca²⁺ sensors. With them, Ca²⁺ levels and distribution in the different embryogenic structures formed during the induction and development of somatic embryos were analyzed by FRET. We also used different chemicals to modulate intracellular Ca²⁺ levels (CaCl₂, ionophore A23187, EGTA), to inhibit calmodulin (W-7) and to inhibit callose synthesis (2-deoxy-D-glucose) at different times, principally during the firs

SUBMITTER: Calabuig-Serna A 

PROVIDER: S-EPMC10102378 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature