Whole-genome sequences from wild-type and laboratory-evolved strains define the alleleome and establish its hallmarks.

ABSTRACT: The genomic diversity across strains of a species forms the genetic basis for differences in their behavior. A large-scale assessment of sequence variation has been made possible by the growing availability of strain-specific whole-genome sequences (WGS) and with the advent of large-scale databases of laboratory-acquired mutations. We define the Escherichia coli "alleleome" through a genome-scale assessment of amino acid (AA) sequence diversity in open reading frames across 2,661 WGS from wild-type strains. We observe a highly conserved alleleome enriched in mutations unlikely to affect protein function. In contrast, 33,000 mutations acquired in laboratory evolution experiments result in more severe AA substitutions that are rarely achieved by natural selection. Large-scale assessme

SUBMITTER: Catoiu EA 

PROVIDER: S-EPMC10104531 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature