Hepatocyte differentiation from mouse liver ductal organoids by transducing 4 liver-specific transcription factors.

ABSTRACT:

Background

Hepatocyte sources that are expandable in vitro are required for liver regenerative medicine and to elucidate the mechanisms underlying the physiological functions of the liver. Liver ductal organoids (LDOs) comprise liver tissue stem cells with a bipotential capacity to differentiate into hepatocyte and cholangiocyte lineages and can thus serve as a hepatocyte source. However, using current differentiation methods, LDOs differentiate into immature hepatocytes while retaining strong cholangiocyte characteristics. We thus investigated an alternative differentiation method for LDOs to achieve hepatocyte maturation.

Methods

We extracted 12 candidate transcription factors to induce hepatocyte differentiation by comparing their gene expression in LDOs and liver tissues

SUBMITTER: Tomofuji K 

PROVIDER: S-EPMC10109133 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature