Hidden in plain sight: challenges in proteomics detection of small ORF-encoded polypeptides.

ABSTRACT: Genomic studies of bacteria have long pointed toward widespread prevalence of small open reading frames (sORFs) encoding for short proteins, <100 amino acids in length. Despite the mounting genomic evidence of their robust expression, relatively little progress has been made in their mass spectrometry-based detection and various blanket statements have been used to explain this observed discrepancy. In this study, we provide a large-scale riboproteogenomics investigation of the challenging nature of proteomic detection of such small proteins as informed by conditional translation data. A panel of physiochemical properties alongside recently developed mass spectrometry detectability metrics was interrogated to provide a comprehensive evidence-based assessment of sORF-encoded polypeptide (SE

SUBMITTER: Fijalkowski I 

PROVIDER: S-EPMC10117744 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature