Protocol to generate murine organotypic brain cultures for drug screening and evaluation of anti-metastatic efficacy.

ABSTRACT: Organotypic brain cultures are short-term assays that phenotypically and functionally recapitulate brain metastatic cells in vivo. Here, we present a protocol to generate murine organotypic brain cultures for drug screening. We describe steps for sectioning of murine brains and plating of organotypic cultures. We then detail evaluation of the anti-metastatic effect of chemical compounds through bioluminescence imaging before and after drug treatment. Combined with downstream applications, this protocol allows comprehensive characterizations of both cancer cells and the tumor-associated microenvironment. For complete details on the use and execution of this protocol, please refer to Zhu et al. (2022).¹.

SUBMITTER: Zhu L 

PROVIDER: S-EPMC10120397 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature