Protocol to generate murine organotypic brain cultures for drug screening and evaluation of anti-metastatic efficacy.
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ABSTRACT:
SUBMITTER: Zhu L
PROVIDER: S-EPMC10120397 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
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Protocol to generate murine organotypic brain cultures for drug screening and evaluation of anti-metastatic efficacy.
Zhu Lucía L Miarka Lauritz L Baena Patricia P Perea-García María M Valiente Manuel M
STAR protocols 20230408 2
Organotypic brain cultures are short-term assays that phenotypically and functionally recapitulate brain metastatic cells in vivo. Here, we present a protocol to generate murine organotypic brain cultures for drug screening. We describe steps for sectioning of murine brains and plating of organotypic cultures. We then detail evaluation of the anti-metastatic effect of chemical compounds through bioluminescence imaging before and after drug treatment. Combined with downstream applications, this p ...[more]
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