Measuring Encapsulation Efficiency in Cell-Mimicking Giant Unilamellar Vesicles.

ABSTRACT: One of the main drivers within the field of bottom-up synthetic biology is to develop artificial chemical machines, perhaps even living systems, that have programmable functionality. Numerous toolkits exist to generate giant unilamellar vesicle-based artificial cells. However, methods able to quantitatively measure their molecular constituents upon formation is an underdeveloped area. We report an artificial cell quality control (AC/QC) protocol using a microfluidic-based single-molecule approach, enabling the absolute quantification of encapsulated biomolecules. While the measured average encapsulation efficiency was 11.4 ± 6.8%, the AC/QC method allowed us to determine encapsulation efficiencies per vesicle, which varied significantly from 2.4 to 41%. We show that it is possible t

SUBMITTER: Supramaniam P 

PROVIDER: S-EPMC10127275 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature