PI3Kα Translocation Mediates Nuclear PtdIns(3,4,5)P₃ Effector Signaling in Colorectal Cancer.

ABSTRACT: The canonical view of PI3Kα signaling describes phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P₃) generation and activation of downstream effectors at the plasma membrane or at microtubule-bound endosomes. Here, we show that colorectal cancer (CRC) cell lines exhibit a diverse plasma membrane-nuclear distribution of PI3Kα, controlling corresponding levels of subcellular PtdIns(3,4,5)P₃ pools. PI3Kα nuclear translocation was mediated by the importin β-dependent nuclear import pathway. By PtdIns(3,4,5)P₃ affinity capture mass spectrometry done in the presence of SDS on CRC cell lines with PI3Kα nuclear localization, we identified 867 potential nuclear PtdIns(3,4,5)P₃ effector proteins. Nuclear PtdIns(3,4,5)P₃ interactome protein

SUBMITTER: Palmieri M 

PROVIDER: S-EPMC10130476 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature