Combination of Recombinant Proteins S1/N and RBD/N as Potential Vaccine Candidates.

ABSTRACT: Despite all successful efforts to develop a COVID-19 vaccine, the need to evaluate alternative antigens to produce next-generation vaccines is imperative to target emerging variants. Thus, the second generation of COVID-19 vaccines employ more than one antigen from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to induce an effective and lasting immune response. Here, we analyzed the combination of two SARS-CoV-2 viral antigens that could elicit a more durable immune response in both T- and B-cells. The nucleocapsid (N) protein, Spike protein S1 domain, and receptor binding domain (RBD) of the SARS-CoV-2 spike surface glycoproteins were expressed and purified in a mammalian expression system, taking into consideration the posttranscriptional modifications and structural chara

SUBMITTER: Mendoza-Ramirez NJ 

PROVIDER: S-EPMC10142685 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature