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Combination of Recombinant Proteins S1/N and RBD/N as Potential Vaccine Candidates.

ABSTRACT: Despite all successful efforts to develop a COVID-19 vaccine, the need to evaluate alternative antigens to produce next-generation vaccines is imperative to target emerging variants. Thus, the second generation of COVID-19 vaccines employ more than one antigen from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to induce an effective and lasting immune response. Here, we analyzed the combination of two SARS-CoV-2 viral antigens that could elicit a more durable immune response in both T- and B-cells. The nucleocapsid (N) protein, Spike protein S1 domain, and receptor binding domain (RBD) of the SARS-CoV-2 spike surface glycoproteins were expressed and purified in a mammalian expression system, taking into consideration the posttranscriptional modifications and structural characteristics. The immunogenicity of these combined proteins was evaluated in a murine model. Immunization combining S1 or RBD with the N protein induced higher levels of IgG antibodies, increased the percentage of neutralization, and elevated the production of cytokines TNF-α, IFN-γ, and IL-2 compared to the administration of a single antigen. Furthermore, sera from immunized mice recognized alpha and beta variants of SARS-CoV-2, which supports ongoing clinical results on partial protection in vaccinated populations, despite mutations. This study identifies potential antigens for second-generation COVID-19 vaccines.

SUBMITTER: Mendoza-Ramirez NJ

PROVIDER: S-EPMC10142685 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Combination of Recombinant Proteins S1/N and RBD/N as Potential Vaccine Candidates.

Mendoza-Ramírez Noe Juvenal NJ García-Cordero Julio J Martínez-Frías Sandra Paola SP Roa-Velázquez Daniela D Luria-Pérez Rosendo R Bustos-Arriaga José J Hernández-Lopez Jesús J Cabello-Gutiérrez Carlos C Zúñiga-Ramos Joaquín Alejandro JA Morales-Ríos Edgar E Pérez-Tapia Sonia Mayra SM Espinosa-Cantellano Martha M Cedillo-Barrón Leticia L

Vaccines 20230418 4

Despite all successful efforts to develop a COVID-19 vaccine, the need to evaluate alternative antigens to produce next-generation vaccines is imperative to target emerging variants. Thus, the second generation of COVID-19 vaccines employ more than one antigen from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to induce an effective and lasting immune response. Here, we analyzed the combination of two SARS-CoV-2 viral antigens that could elicit a more durable immune response in bo ...[more]

PMID: 37112776

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Combination of Recombinant Proteins S1/N and RBD/N as Potential Vaccine Candidates.

Publications

Combination of Recombinant Proteins S1/N and RBD/N as Potential Vaccine Candidates.

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