Skeletal muscle mitochondrial interactome remodeling is linked to functional decline in aged female mice.

ABSTRACT: Genomic, transcriptomic and proteomic approaches have been used to gain insight into molecular underpinnings of aging in laboratory animals and in humans. However, protein function in biological systems is under complex regulation and includes factors besides abundance levels, such as modifications, localization, conformation and protein-protein interactions. By making use of quantitative chemical cross-linking technologies, we show that changes in the muscle mitochondrial interactome contribute to mitochondrial functional decline in aging in female mice. Specifically, we identify age-related changes in protein cross-links relating to assembly of electron transport system complexes I and IV, activity of glutamate dehydrogenase, and coenzyme-A binding in fatty acid β-oxidation and tricarbox

SUBMITTER: Bakhtina AA 

PROVIDER: S-EPMC10154043 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature