Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures.

ABSTRACT: Recent advances in synthetic embryology have opened new avenues for understanding the complex events controlling mammalian peri-implantation development. Here, we show that mouse embryonic stem cells (ESCs) solely exposed to chemical inhibition of SUMOylation generate embryo-like structures comprising anterior neural and trunk-associated regions. HypoSUMOylation-instructed ESCs give rise to spheroids that self-organize into gastrulating structures containing cell types spatially and functionally related to embryonic and extraembryonic compartments. Alternatively, spheroids cultured in a droplet microfluidic device form elongated structures that undergo axial organization reminiscent of natural embryo morphogenesis. Single-cell transcriptomics reveals various cellular lineages, including pr

SUBMITTER: Cossec JC 

PROVIDER: S-EPMC10157296 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature