USF1/CD90 signaling in maintaining glioblastoma stem cells and tumor-associated macrophages adhesion.

ABSTRACT:

Background

Glioblastoma stem cells (GSCs) and their interplay with tumor-associated macrophages (TAMs) are responsible for malignant growth and tumor recurrence of glioblastoma multiforme (GBM), but the underlying mechanisms are largely unknown.

Methods

Cell viability, stemness, migration, and invasion were measured in GSCs after the knockdown of upstream stimulating factor 1 (USF1). Luciferase assay and chromatin immunoprecipitation qPCR were performed to determine the regulation of CD90 by USF1. Immunohistochemistry and immunofluorescent staining were used to examine the expression of USF1 and GSC markers, as well as the crosstalk between GSCs and TAMs. In addition, the interaction between GSCs and TAMs was confirmed using in vivo GBM models.

Results

We show that US

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC10167418 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature