Single-cell RNA sequencing reveals the local cell landscape in mouse epididymal initial segment during aging.

ABSTRACT:

Background

Morphological and functional alterations in aging reproductive organs result in decreased male fertility. The epididymis functions as the transition region for post-testicular sperm maturation. And we have previously demonstrated that the epididymal initial segment (IS), a region of the reproductive tract essential for sperm maturation and capacitation, undergoes considerable histological changes and chronic immune activation in mice during aging. However, the local aging-associated cellular and molecular changes in the aged epididymal IS are poorly understood.

Results

We conducted single-cell RNA sequencing analysis on the epididymal IS of young (3-month-old) and old (21-month-old) mice. In total, 10,027 cells from the epididymal IS tissues of young and old mice

SUBMITTER: Zhuang J 

PROVIDER: S-EPMC10173474 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature