Dataset Information

Bioinformatic analysis identifies GPR91 as a potential key gene in brain injury after deep hypothermic low flow.

ABSTRACT:

Purpose

Explore the transcription change of brain ischemia and reperfusion injury after deep hypothermic low flow.

Method

The data from PRJNA739516 and GSE104036 were obtained for the differentially expressed genes identification, functional enrichment analysis, gene set enrichment analysis, protein-protein interaction construction and hub gene identification. Oxygen and glucose deprivation model was set to validate the hub gene and explore the detailed brain injury mechanism.

Result

Interleukin, immunological response, NF-κB signaling pathway, G protein-coupled receptor signaling pathway and NLRP inflammatory are functional pathway were enriched in differentially expressed genes analysis. Sucnr1, Casr, Cxcr4, C5ar1, Tas2r41, Tas2r60 and Hcar2 were identified and verified in the OGD model. Knocking down GPR91 reduces the inflammatory response after OGD and GPR91 may be involved in the inflammatory pre-reaction through the synergistic activation of NF-κB, NLRP3, and IL-1β respectively.

Conclusion

Our study found that Interleukin, immunological response, NF-κB signaling pathway, G protein-coupled receptor signaling pathway and NLRP inflammatory are all associated with brain ischemia and reperfusion injury after deep hypothermic low flow and GPR91 can activate NF-κB/NLRP3 pathway and trigger the release of IL-1β in this progress.

SUBMITTER: Puwei S

PROVIDER: S-EPMC10176032 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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Publications

Bioinformatic analysis identifies GPR91 as a potential key gene in brain injury after deep hypothermic low flow.

Puwei Song S Jiali Xu X Zhuoga Deqin D Kede Wu W Patel Nishant N Jia An A Jirong Qi Q Xuming Mo M

Heliyon 20230415 5

<h4>Purpose</h4>Explore the transcription change of brain ischemia and reperfusion injury after deep hypothermic low flow.<h4>Method</h4>The data from PRJNA739516 and GSE104036 were obtained for the differentially expressed genes identification, functional enrichment analysis, gene set enrichment analysis, protein-protein interaction construction and hub gene identification. Oxygen and glucose deprivation model was set to validate the hub gene and explore the detailed brain injury mechanism.<h4> ...[more]

PMID: 37187908

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Dataset Information

Bioinformatic analysis identifies GPR91 as a potential key gene in brain injury after deep hypothermic low flow.

Purpose

Method

Result

Conclusion

Publications

Bioinformatic analysis identifies GPR91 as a potential key gene in brain injury after deep hypothermic low flow.

Similar Datasets

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