Bioinformatic analysis identifies GPR91 as a potential key gene in brain injury after deep hypothermic low flow.

ABSTRACT:

Purpose

Explore the transcription change of brain ischemia and reperfusion injury after deep hypothermic low flow.

Method

The data from PRJNA739516 and GSE104036 were obtained for the differentially expressed genes identification, functional enrichment analysis, gene set enrichment analysis, protein-protein interaction construction and hub gene identification. Oxygen and glucose deprivation model was set to validate the hub gene and explore the detailed brain injury mechanism.

Result

Interleukin, immunological response, NF-κB signaling pathway, G protein-coupled receptor signaling pathway and NLRP inflammatory are functional pathway were enriched in differentially expressed genes analysis. Sucnr1, Casr, Cxcr4, C5ar1, Tas2r41, Tas2r60 and Hcar2 were identified and veri

SUBMITTER: Puwei S 

PROVIDER: S-EPMC10176032 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature