Rapid and Simple Detection of Burkholderia gladioli in Food Matrices Using RPA-CRISPR/Cas12a Method.

ABSTRACT: Pathogenic variants of Burkholderia gladioli pose a serious threat to human health and food safety, but there is a lack of rapid and sensitive field detection methods for Burkholderia gladioli. In this study, the CRISPR/Cas12a system combined with recombinant enzyme polymerase amplification (RPA) was used to detect Burkholderia gladioli in food. The optimized RPA-CRISPR/Cas12a assay was able to specifically and stably detect Burkholderia gladioli at a constant 37 °C without the assistance of large equipment. The detection limit of the method was evaluated at two aspects, the genomic DNA (gDNA) level and bacterial quantity, of which there were 10^-3 ng/μL and 10¹ CFU/mL, respectively. Three kinds of real food samples were tested. The detectio

SUBMITTER: Zheng J 

PROVIDER: S-EPMC10178126 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature