A high-concentrate diet induces inflammatory injury via regulating Ca²⁺/CaMKKβ-mediated autophagy in mammary gland tissue of dairy cows.

ABSTRACT:

Introduction

Calmodulin-dependent protein kinase β (CaMKKβ) is closely related to Ca²⁺ concentration. An increase in Ca²⁺ concentration in the cytoplasm activates CaMKKβ, and activated CaMKKβ affects the activities of AMPK and mTOR and induces autophagy. A high-concentrate diet leads to Ca²⁺ disorder in mammary gland tissue.

Objectives

Therefore, this study mainly investigated the induction of mammary gland tissue autophagy by a high-concentrate diet and the specific mechanism of lipopolysaccharide (LPS)-induced autophagy in bovine mammary epithelial cells (BMECs).

Material and methods

Twelve mid-lactation Holstein dairy cows were fed with a 40% concentrate diet (LC) and a 60% concentrate diet (HC) for 3 weeks. At the end of the trial,

SUBMITTER: Meng M 

PROVIDER: S-EPMC10183583 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature