Novel CH25H⁺ and OASL⁺ microglia subclusters play distinct roles in cerebral ischemic stroke.

ABSTRACT:

Background

Microglial polarization is one of the most promising therapeutic targets for multiple central nervous system (CNS) disorders, including ischemic stroke. However, detailed transcriptional alteration of microglia following cerebral ischemic stroke remains largely unclear.

Methods

Focal cerebral ischemia was induced by transient middle cerebral artery occlusion (tMCAO) for 60 min in mice. Single-cell RNA sequencing (scRNA-seq) was performed using ischemic brain tissues from tMCAO and sham mice 3 days after surgery. Ch25h^-/- mice were used to investigate the role of specific microglia subcluster on post-stroke infarct volume and neuroinflammation.

Results

We identified a relatively homeostatic subcluster with enhanced antigen processing and three "is

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10184422 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature