GPX8 deficiency-induced oxidative stress reprogrammed m6A epitranscriptome of oral cancer cells.

ABSTRACT: Glutathione peroxidase 8 (GPX8) is a key regulator of redox homoeostasis. Whether its antioxidant activity participates in the regulation of m⁶A modification is a crucial issue, which has important application value in cancer treatment. In this study, MeRIP-seq was used to explore the characteristics of transcriptome-wide m⁶A modification in GPX8-deficient oral cancer cells. Oxidative stress caused by the lack of GPX8 resulted in 1,279 hyper- and 2,287 hypo-methylated m⁶A peaks and 2,036 differentially expressed genes in GPX8-KO cells. Twenty-eight differentially expressed genes were related to the cell response to oxidative stress, and half of them changed their m⁶A modification. In GPX8-KO cells, m⁶A regulators IGF2BP2 and IGF2BP3 we

SUBMITTER: Chen X 

PROVIDER: S-EPMC10184595 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature