Characterization of bla_AFM-1-positive carbapenem-resistant strains isolated in Guangzhou, China.

ABSTRACT:

Background

Carbapenemase-producing makes a great contribution to carbapenem resistance in Gram-negative bacilli. Bla_AFM-1 gene was first discovered by us in Alcaligenes faecalis AN70 strain isolated in Guangzhou of China and, was submitted to NCBI on 16 November 2018.

Methods

Antimicrobial susceptibility testing was performed by broth microdilution assay using BD Phoenix 100. The phylogenetic tree of AFM and other B1 metallo-β-lactamases was visualized by MEGA7.0. Whole-genome sequencing technology was used to sequence carbapenem-resistant strains including the bla_AFM-1 gene. Cloning and expressing of bla_AFM-1 were designed to verify the function of AFM-1 to hydrolyze carbapenems and common β-lactamase substrates. Carba NP and Etest experimen

SUBMITTER: Qin Y 

PROVIDER: S-EPMC10189940 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature