Deconvolution of spatial sequencing provides accurate characterization of hESC-derived DA transplants in vivo.

ABSTRACT: Cell therapy for Parkinson's disease has experienced substantial growth in the past decades with several ongoing clinical trials. Despite increasing refinement of differentiation protocols and standardization of the transplanted neural precursors, the transcriptomic analysis of cells in the transplant after its full maturation in vivo has not been thoroughly investigated. Here, we present spatial transcriptomics analysis of fully differentiated grafts in their host tissue. Unlike earlier transcriptomics analyses using single-cell technologies, we observe that cells derived from human embryonic stem cells (hESCs) in the grafts adopt mature dopaminergic signatures. We show that the presence of phenotypic dopaminergic genes, which were found to be differentially expressed in the transp

SUBMITTER: Rajova J 

PROVIDER: S-EPMC10209706 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature