VEGF overexpressed mesoangioblasts enhance urethral and vaginal recovery following simulated vaginal birth in rats.

ABSTRACT: Vaginal birth causes pelvic floor injury which may lead to urinary incontinence. Cell therapy has been proposed to assist in functional recovery. We aim to assess if intra-arterial injection of rat mesoangioblasts (MABs) and stable Vascular Endothelial Growth Factor (VEGF)-expressing MABs, improve recovery of urethral and vaginal function following simulated vaginal delivery (SVD). Female rats (n = 86) were assigned to either injection of saline (control), allogeneic-MABs (MABs^allo), autologous-MABs (MABs^auto) or allogeneic-MABs transduced to stably expressed VEGF (MABs^allo-VEGF). One hour after SVD, 0.5 × 10⁶ MABs or saline were injected into the aorta. Primary outcome was urethral (7d and 14d) and vaginal (14d) function; others were bioluminesc

SUBMITTER: Mori da Cunha MGMC 

PROVIDER: S-EPMC10224946 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature